Materials and Methods

 

• Materials and Laboratory Apparatus
• Chemicals
• Buffers
  • Stock Solutions
  • Other Solutions
• Culture Media
  • Liquid Media
  • Solid Media
  • Antibiotics
  • Top-Agar
• General Methods
  • DNA Preparation from Whole Blood
  • Nucleic Acid Quantification
  • Restriction Endonucleases and Their Use
  • Phenol Extraction
  • Ethanol Precipitation
  • Oligonucleotide Purification
  • Heat Denaturation of DNA
• PCR Techniques
  • Standard PCR Conditions
  • Primer Selection
  • Determination of Melting Temperatures
  • The PCR Buffer
  • Factors Affecting Specifity
  • Fidelity of Amplification
  • Used Materials and Precautions
  • Oligonucleotide Quantification
• Gel Electrophoresis
  • Agarose Gel Electrophoresis
  • Freeze-Squeeze-Technique
  • Polyacrylamide Gels