The following method was used to make hard agar-based culture plates for growth
of bacterial colonies.
To obtain solid media, 15 g agar were added to the liquid medium (LB) before
autoclavation. After cooling to 50psy176 C, the agarose-medium was poured
directly into petri dishes, allowing about 30-35 ml per 85mm dish. To avoid
bubbles, the surface of the medium was flamed with a bunsen burner before the
gel hardened.