Four of the sequenced mutations (1309, 1515, 1786 and 2316,
see section results) were scanned by RFLP using agarose as well as
polyacrylamide gels. Most of the samples used were scrutinized in previous
studies, and a phenotype ( 
receptor density) was available.
RFLP results for both, agarose minigels with ethidiumbromide staining, as well
as polyacrylamide gels with silver staining, are given below. The marker used
for agarose gels was Biomarker Low (BioVentures, Inc.), showing bands at 100,
200, 300, 400, 500, 525, 700 and 1000 base pairs. The marker used for
non-denaturing polyacrylamide gels (CleanGels) was the 100bp ladder
(Pharmacia). Note, that in non-denaturing gels the mobilities of DNA fragments
are also dependent on the base sequence (content of A and T). This can be
easily seen in figures fig:cg_bfa and fig:cg_kpn.
Figure 4.3: RFLP for position 1309 (agarose gel)
Lane M: Marker; Lanes 1, 4: heterozygous; Lane 2: homozygous G;
Lane 3: homozygous A
Adrenergic Receptor Allele Frequencies and Haplotypes
Adrenoceptor Alleles in Relation to Adrenoceptor
Expression in Cultured Skin Fibroblasts
