10 mg/ml. Used to
visualise DNA: emits fluorescence when excited at
250–320nm (for formula, see figure 2.1). Stored at
4℃C in dark.
Proteinase K:
30 mg Proteinase K are dissolved in 1ml H2O and
stored at –20℃C.
TE buffer:
10 mM Tris (pH 7.4) and 0.1 mM EDTA–Na2 (pH
8.0), stored at room temperature.
Sevac:
24 parts chloroform mixed with 1 part isoamyl
alcohol.
NaCl, 6M:
35.06 g NaCl are dissolved in 100 ml H2O and
stored at 4℃C.
PAGE loading buffer:
5 parts of deionised formamide and 1 part of
25mM EDTA (pH 8.0) with 50 mg/ml blue
dextran.
Phenol:
Phenol
was redistilled at 160℃C and 8-hydroxyquinoline
added to a final concentration of 0.1%. It was then
extracted several times with an equal volume of
buffer (1M Tris [pH 8.0], followed by 0.1M Tris
[pH 8.0] and 0.2% β-mercaptoethanol). Stored at
4℃C in dark under equilibration buffer.
Deionised Formamide:
50ml Formamide1
are mixed with 5 g of AG501 X8 ion-exchange resin
and stirred for 30 minutes. This is repeated until
the pH is greater than 7.0. The formamide is dispensed
into aliquots of 500 µl and stored for up to 3 months
at -25℃C.