2.3.1 Stock Solutions

APS, 10%:

0.5 g ammonium persulfate dissolved in 5 ml water, stored in 100 µl aliquots at -25℃C.

EDTA, 0.5M:

186 g/l ethylenediamine tetraacetic acid, disodium salt and 10 N NaOH to pH 8.0, autoclaved.

Glucose, 20%:

20 g glucose dissolved in 100ml water, sterile filtered.

3M KAc/5M HAc:

29.4 g potassium acetate and 11.5 ml acetic acid in 100 ml of water. Autoclaved.

NaAc, 3M:

408 g CH₃COONa ⋅ 3H₂O in 1 litre H₂O. Acetic acid to pH 5.2, autoclaved.

SDS 20%:

200 g SDS (Sodium dodecyl sulfate) dissolved in 1 litre water at 60℃C, not autoclaved!

TAE buffer, 40Õ:

193.6 g Tris-base, 15.2 g EDTA-Na₂⋅ 2H₂O, 108.9 g Na acetate ⋅ 3H₂O. Acetic acid to pH 7.2, H₂O to make 1 litre. Stored at room temperature.

TBE buffer, 10Õ:

60.5 g Tris-base, 30.8 g boric acid and 3.72 g EDTA-Na₂ ⋅ 2H₂O, H₂O to make 1 litre. Stored at room temperature. Remade if major residues appear in solution.

Lysis buffer 10Õ:

82.9 g NH₄Cl, 10 g KHCO₃ and 0.37 g EDTA-Na₂ ⋅ 2H₂O, H₂O to make 1 litre. pH adjusted to 7.4, autoclaved. Stored at 4℃C.

Gel loading buffer, 5Õ:

50% glycerol, 0.5% bromophenol blue, 0.5% xylene cyanol in 5Õ TAE.

© 2001 Alexander Binder