10Õ TBE and deionised water (Millipore) was used for the ABI377 buffer tanks. Samples were loaded in alternate wells, and run into the gels using a short (5min) prerun.
All gels were run using 1200 scans/hr run modules. Run time for 36-cm gels was 5 - 7 hours (depending on PCR fragment length), for 48-cm gels 8 - 10 hours. Prerun time was between 10 and 20 minutes. For 96 well gels, a different machine protocol (resulting in more readable channels) was used. Run temperature was 51℃C. Sequence analysis was done with PE Biosystems Sequence Analysis 3.4 software. Correct lane tracking was checked manually.
© 2001 Alexander Binder