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2.5.8 Oligonucleotide Quantification

The molar concentration of the used oligonucleotide primers was determined by reading the absorption of a 1:100 diluted sample at 260 nm. The molar extinction coefficient was calculated using the following per nucleotide coefficients:

N
ε260 [cm2/mol]
T 8400
A 15200
G 12010
C 7000

Table 2.1: Molar extinction coefficients of nucleotides

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© 2001 Alexander Binder