Plasticware. All experiments were performed in lockable sterilised polypropylene test tubes (capacity of 0.2 to 50 ml). Liquids were transferred by automatic pipettors (Gilson) using sterilised pipette-tips. For volumes larger than 1 ml, sterilised graduated plastic pipettes were used. All tubes were sterilised by steam autoclaving (45min, 121℃C).
Centrifuges. Centrifugations were run in cooled or unrefrigerated microcentrifuges (Eppendorf tubes) and refrigerated tabletop centrifuges.
Refrigeration. A refrigerator and a –20℃C freezer were used. A refrigerated cabinet (4℃C) was used for microcentrifugation. Liquid nitrogen and crashed ice was also necessary for this study.
Light measurement. A UV/Vis spectrophotometer was used for DNA quantification. An ultraviolet (UV) transilluminating light source (302 nm) was necessary to visualise reaction products. A dark room equipped with a Polaroid camera and a developing unit was used for documentation.
Electrophoresis systems. Two power supplies and two different gel-systems (vertical sequencing unit and submerged minigels) were used together with the necessary auxiliary equipment.
Thermocycler. An OmniGene (Hybaid) as well as a GeneAmp PCR System 9700 (Perkin-Elmer) thermocycler were used during this study.
Common laboratory equipment. A pH meter, hotplate-stirrer, vortex mixer, balances for weighting reagents, laboratory timers and desiccators were required.
© 2001 Alexander Binder