Other Solutions

Xgal, 2%:
2mg Xgal  (5-bromo-4-chloro-3-indolyl- -D-galactoside,   for formula see figure fig:xgal) in 1 ml of dimethylformamide  (DMF ). Stored dark at -20psy176 C.
IPTG, 100mM:
23.8 mg isopropyl- -D-thiogalactopyranoside (for formula, see figure fig:iptg)     in 1 ml H O, stored at -20psy176 C.
Ethidium bromide solution:
10mg/ml. Used to visualize DNA: emits fluorescence when excited at 250-320nm (for formula, see figure fig:etbr). Stored at 4psy176 C in dark. Handle with gloves and avoid inhalation. 
Proteinase K:
30mg Proteinase K  are dissolved in 1ml H O and stored at -20psy176 C.
Lysis buffer I:
50 mM glucose, 25 mM Tris-HCl and 10 mM EDTA. pH adjusted to 8.0, stored at 4psy176 C.
Lysis buffer II:
0.2 M NaOH and 1% SDS in water, always prepared freshly.
TE buffer:
10 mM Tris (pH 7.4) and 0.1 mM EDTA-Na (pH 8.0), stored at room temperature.
Sevac :
24 parts chloroform mixed with 1 part isoamyl alcohol .
Phenol:
Phenol  was redistilled at 160psy176 C and 8-hydroxyquinoline  added to a final concentration of 0.1%. It was then extracted several times with an equal volume of buffer (1M Tris [pH 8.0], followed by 0.1M Tris [pH 8.0] and 0.2% -mercaptoethanol) . Stored at 4psy176 C in dark under equilibration buffer.